HIGHLIGHTS
SUMMARY
To better define viable probiotic and CFS activity in a complex bacterial environment, the same experiments were performed combining all three pathogens, also developing an auto- and co-aggregation assay, to determine whether live probiotics and their CFSs could interfere with pathogen interactions. In general, although the containment effect of viable probiotic strains was milder, L rhamnosus LR04 and L casei LC04 completely inhibited the three-pathogen co-culture growth, while L reuteri LRE11 CFS was still the best in reducing the three-pathogen viability. Developing these assays in a pathogen co-culture environment . . .
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