Inducible expression of large grna arrays for multiplexed crisprai applications

HIGHLIGHTS

• who: William M. Shaw from the (UNIVERSITY) have published the research work: Inducible expression of large gRNA arrays for multiplexed CRISPRai applications, in the Journal: (JOURNAL) of 13/Oct/2021
• what: The authors develop a method for near leak-free, inducible expression of a polycistronic array containing up to 24 gRNAs from two orthogonal CRISPR/Cas systems to increase CRISPRai multiplexing capacity and target gene flexibility. The authors demonstrate this toolkit by targeting 11 genes in central metabolism in a single transformation, achieving a 45-fold increase in succinic acid, which could be precisely controlled . . .
  

   

  

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