Inducible high-efficiency crispr-cas9-targeted gene editing and precision base editing in african trypanosomes

HIGHLIGHTS

  • who: from the (UNIVERSITY) have published the research work: Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes, in the Journal: (JOURNAL) of 23/02/2018
  • how: To determine whether the efficiency of the system could be extended to another native locus and to further test sgRNA design for T. brucei the authors selected a second gene on a different chromosome for analysis.

SUMMARY

    This allows for selection of hygromycin-resistant clones with expression cassettes reproducibly integrated at the same locus, a locus that supports . . .

     

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