Induction of antitoxic antibody and preventive effect against porcine edema disease by the pentameric stx2eb subunit vaccine

HIGHLIGHTS

SUMMARY

ED is an enterotoxaemia caused by Stx2e that frequently occurs in 4-12 week-old piglets. The nucleotide sequence for a fusion_protein of_(GP)2GH6(G4S)3 linker and COMP was amplified by PCR using a plasmid pB-spacer which includes nucleotide sequence for ­ (G4S)3-COMP and primers (sense, 5′-GGG​CTC​GAG​GGT​CCA​GGA​CCT​GGA​ CATC-3′ and antisense, 5′-GGG​CTC​GAG​TCA​GCC​ CGG​GGT​ACG​GGCC-3′). The pSTXC plasmid is a vector for expressing a fusion_protein of Stx2eB, the (GP)2GH6(G4S)3 linker, and COMP . . .

 



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