Induction of functional xeno-free mscs from human ipscs via a neural crest cell lineage

HIGHLIGHTS

• who: Daisuke Kamiya from the , (Hs00984148_m1) and PD-, (Hs00204257_m1) expressions were measured by TaqMan Gene Expression Assays (Thermo Fisher Scientific)Transcriptome analysis Total RNA was purified using the RNeasy Micro Kit (Qiagen) and treated using the DNase-one kit (Qiagen) to remove genomic DNA. We reverse transcribed , ng of total RNA to obtain singlestranded cDNA using the SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific). We performed cDNA library synthesis for the Ion Ampliseq transcriptome using the Ion AmpliSeq Transcriptome Human Gene Expression Core Panel (Thermo Fisher Scientific) and the Ion Ampliseq Library Kit Plus (Thermo Fisher . . .
  

   

  

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