HIGHLIGHTS
- who: Jian Ma and collaborators from the cells were prepared from EIAV negative fetal donkeys and cultured in minimal essential medium (α-MEM, Gibco) containing , mM, glutamine, % heat-inactivated FBS, IU penicillin, and , μg/mL streptomycin (Gibco) as previously described [10]Two EIAV strains were used in this study. EIAVFDDV13, an attenuated vaccine strain of EIAV, was developed by passaging EIAVDLV121, a Chinese donkey leukocyte-adapted attenuated strain of EIAV, in FDD cells for , generations. A protective test demonstrated that , induced protection from disease in approximately, % of vaccinated horses [7], and the strain remained stably attenuated in the . . .
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