Infrared spectroscopy elucidates the inhibitor binding sites in a metal‐dependent formate dehydrogenase

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  • who: from the (UNIVERSITY) have published the Article: Infrared Spectroscopy Elucidates the Inhibitor Binding Sites in a Metalu2010Dependent Formate Dehydrogenase, in the Journal: (JOURNAL)
  • what: By means of a combined approach including inhibition kinetics, site directed mutagenesis, DFT calculations and IR spectroscopy, we have elucidated the binding motifs of inhibitory azide and cyanate anions in the Mo-dependent formate dehydrogenase.
  • how: In this study infrared (IR) spectroscopy was employed in combination with density functional theory (DFT) and inhibition kinetic studies to elucidate the specific interaction of such inhibitory CO2 analogues with RcFDH.
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