Inhibition of the pcr by genomic dna

HIGHLIGHTS

  • who: Sue Latham and colleagues from the College of Medicine and Public Health, Flinders University, Adelaide, Australia have published the article: Inhibition of the PCR by genomic DNA, in the Journal: PLOS ONE of November/14,/2022
  • how: All PCRs were quantitative PCRs performed using a Biorad CFX Connect Real-time PCR detection system and the results were analysed using Biorad CFX Manager 3.1 software. Concomitantly the primer at 900 nmol/l was tested for inhibition of the plasmid test system with 3 copies of the plasmid target in each well.

SUMMARY . . .

 

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