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- who: Charlotte LeKieffre from the Preparation for TEM-NanoSIMS analysisThe specimens were chemically fixed following the protocol described44). Briefly, foraminifera were fixed immediately after removal from the incubator, with a mix of, % glutaraldehyde and, % paraformaldehyde diluted in, ., M cacodylate buffer, ., M sucrose, and, ., M NaCl (pH=7.4) at room temperature for , h. They were then stored at , °C until further processing. Further chemical processing and transmission electron microscope (TEM) imaging of the foraminifera were performed at the Electron Microscopy Facility of the University of Lausanne (Switzerland). After rinsing, specimens were SCiENtifiC RepOrts have published the research . . .
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