Insights into receptor structure and dynamics at the surface of living cells

HIGHLIGHTS

• who: Frederik Steiert from the , , , , ]) were derived based on IL Ru03b1* by site-directed mutagenesis (GeneArt, Invitrogen)Note that, due to the His-tag, numbering is shifted by +, amino acids with respect to mature wild-type. In addition, for a second construct of receptor mutant K B the, terminal eGFP was removed (pCMV_H IL Ram266-K B) for negative controls in FCCS and dual-channel BCRI. While the CMV-promotor was necessary for the GCE-mediated expression of receptor mutants (pCMV_H6-IL Ram266-X>B-eGFP), microscopy required to express the non-modified control IL- R* under . . .
  

   

  

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