HIGHLIGHTS
- who: Sophie M. Travis from the Purification of augmin complexes and subcomplexes was adapted from a previously described protocol, L infected Sf, cells (for, II and, III subcomplexes) or , L cells (for octameric augmin) were harvested at, x g and resuspended in lysis buffer (, mM Tris pH, .7, mM NaCl, mM EDTA, mM u03b2-mercaptoethanol, % glycerol, .05% Tween, ) supplemented with , ug/mL DNase I (Roche) and , cOmplete protease inhibitor cocktail tablet (Roche). After lysis via Emulsiflex, lysates were clarified at, g and loaded onto either , mL StrepTactin Superflow (IBA), for, III, or , mL IgG Sepharose (Cytiva), for . . .
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