Interaction of aryl hydrocarbon receptor-interacting protein-like 1 with the farnesyl moiety*

HIGHLIGHTS

• who: Anurima Majumder from the model using supcomb, (41) and visualized in PyMOL, .0., (The PyMOL Molecular Graphics System, Schrödinger, LLC). Preparation of the Fluorescence Probe Farnesyl-Cys-AMCA- Conjugation of AMCA-X to cysteine farnesyl methyl ester was performed at , °C for , h using, respectively, mM and , mM solutions of the reagents in, % acetonitrile, % dimethylformamide, and , mM HEPES (pH, .3). The product, farnesylCys-AMCA, was purified by reverse-phase HPLC. Cys-AMCA was prepared by reacting , mM AMCA-X with , mM cysteine in , mM HEPES (pH, .5), % acetonitrile for , min at , °C. Fluorescence Assay-Fluorescence . . .
  

   

  

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