Interactions between src homology (sh) 2/sh3 adapter proteins and the guanylnucleotide exchange factor sos are differentially regulated by insulin and epidermal growth factor*

HIGHLIGHTS

• who: Shuichi Okada from the Cell Culture-CHO cells expressing both the human insulin and EGF receptors were obtained as described previously (35)These cells were maintained in minimal Eagle's medium containing nucleotides plus, % fetal bovine serum. The cells were grown to confluence and incubated in serum-free medium for , h prior to the addition of , nM insulin or , nM EGF at , °C for the times indicated in the figure legends. Western Blotting and Immunoprecipitation-Whole cell extracts were prepared by detergent solubilization in a lysis buffer (, mM Hepes, pH, .4, % Triton, mM MgCl2, mM EDTA . . .
  

   

  

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