Intermediate domain of receptor-interacting protein kinase 1 (ripk1) determines switch between necroptosis and ripk1 kinase-dependent apoptosis*

HIGHLIGHTS

• who: Linde Duprez from the Antibodies, Cytokines, and Reagents-Recombinant human TNF was produced in Escherichia coli and purified to at least, % homogeneity in our laboratoriesThe specific biological activity was , ⫻ , IU/ml as determined in a standardized cytotoxicity assay on, sA cells. The caspase peptide inhibitor, VAD-fmk (Bachem, Bubendorf, Switzerland) was used at , ␮M., Diphenyltetrazolium bromide (Sigma Aldrich) was used at , mg/ml. Nec, (Calbiochem, San Diego, CA) was used at , ␮M. Propidium iodide (Sigma Aldrich) was used at , ␮M. The following antibodies were used for, sA cells: antic, (, antibody (35), a kind gift from Dr . . .
  

   

  

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