Isoform- and ligand-specific modulation of the adhesion gpcr adgrl3/latrophilin3 by a synthetic binder

HIGHLIGHTS

• who: Szymon P. Kordon from the Cloning, overexpression and purification of sABs Phage ELISA results were used to select sAB clones that were sequenced at DNA Sequencing Facility at The University of ChicagoInfusion cloning, was used to reformat unique sABs clones into pRH2.2, an IPTG inducible vector for bacterial expression. E. coli , (Gold) cells were transformed with sequence-verified sAB plasmids. Cultures were grown in YT media supplemented with , u03bcg m, of ampicillin at , u00b0C until they reached OD600=0.8, when Nature Communications have published the article: Isoform- and ligand-specific modulation of the adhesion . . .
  

   

  

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