Isolation and characterization of apical papilla cells from root end of human third molar and their differentiation into cementoblast cells: an in vitro study

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  • who: Morvarid Ebadi from the All research protocol of this study were approved by the Ethics Committee of Islamic Azad University, Central Tehran Branch (CoIR.IAU.CTB.REC.1400.110). The tooth roots of molar teeth which extracted due to orthodontic treatment were harvested for stem cells. The apical part of the extracted tooth was rinsed with phosphatebuffered saline (PBS) and then stored and incubated in a digestion solution containing , mL of PBS and , mg/mL of type II collagen at , u00b0C for , h. After centrifuging, the resulting solution was transferred into a , flask containing DMEM-, medium . . .

     

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