Isolation, characterization, and autophagy function of becn1-splicing isoforms in cancer cells

HIGHLIGHTS

SUMMARY

    Human Beclin 1 (BECN1) is a haploinsufficient tumor suppressor gene located on chromosome 17q21 breast cancer tumor susceptibility locus in close proximity to BRCA1. (B) Multiple sequence alignment of the amino_acid sequences of the BECN1 isoforms (wt, α, β, and γ). In the isoforms BECN1-β and BECN1-γ, the deletion of 229 nt results in the removal of 76 amino_acids (amino_acids 86-163) forming the BH3D and part of the CCD. The second deletion of 205 nt in the isoform BECN1-β results in the loss of additional 68 amino_acids but does not introduce a premature STOP codon. As . . .

     

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