Isotope tracing reveals distinct substrate preference in murine melanoma subtypes with differing anti-tumor immunity

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SUMMARY

    Most investigations into the role of glutamine have focused on the impact of glutamine metabolism in TILs in_vitro, while largely ignoring the impact of glutamine on tumor cell division and function in the in_vivo tumor microenvironment (TME). The study seeks to combine in_vivo and in_vitro studies implanting ­[13C] tracer methodology combined with gas and liquid chromatography-based analysis to address these gaps. Most of these studies, however, were conducted in an in_vitro T_cell culture system and lack in_vivo evidence to confirm the physiologic impact of T_cell metabolism on tumor cells in the TME. Unlike . . .

     

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