HIGHLIGHTS
- who: ADHI from the Western Blot Analysis Proteins were extracted from control, PGPR treated and challenged with Ccapsici turmeric leaves by grinding , g of tissues with , ml of, ., M phosphate buffer, pH, ., containing, ., mM phenylmethylsulphonyl fluoride as described by Velazhahan et_al (1998). Proteins (, u03bcg) in aliquots of extracts were separated on, % SDS-PAGE [22] and electroblotted onto polyvinylene difluoride (PVDF) membrane (pore sizeu03bcm, Bio- Rad) using BioRad semidry transfer cell in accordance with the manufacturer`s instructions. Western blotting was carried out as described by [24] Winston et al. (1987) with an antiserum raised against barley chitinase . . .
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