Jak/stat3 regulated global gene expression dynamics during late-stage reprogramming process

HIGHLIGHTS

• who: Ling Wang from the Total RNA was isolated from reprogrammed cells with different treatments using RNeasy Mini kit (Qiagen, Valencia, CA) and reverse transcribed using the SuperScript III Reverse Transcription Kit (Invitrogen, Grand Island, NY)The quality of Total RNA was examined with the Aglient RNA , Nano kit (Aglient Technologies, Santa Clara, CA). rRNA was then removed by using Ribo-Zero-rRNA Removal kit (Epicentre, Madison, WI). , ng of rRNA-depleted total RNA from each sample was used to prepare the RNA sequencing library following the manufacturer's instructions by SOLiD Total RNA-seq Kit (Life . . .
  

   

  

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