Jumonji, a critical factor for cardiac development, functions as a transcriptional repressor*

HIGHLIGHTS

• who: Tae-Gyun Kim from the flanked by , bases of nonrandom sequence, ⬘-GCGAAGCTTTGACTGAGGN TTGATGCCGAGGATCCCG-3⬘ (Biotechnology Center at the University Of Wisconsin)BamHI and HindIII sites are underlined. The PCR primers annealing to the , bases of each top and bottom strand were synthesized. The, ⬘ primer was used for a primer extension reaction to make double-stranded oligonucleotides, which was purified by PAGE. The selection procedure was initiated by mixing , ␮g of the purified double-stranded random oligonucleotides with , ng of GSTJMJ coupled to glutathione-agarose beads in buffer A (0., ml of , mM Tris, pH, .0, mM . . .
  

   

  

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