“just right” combinations of adjuvants with nanoscale carriers activate aged dendritic cells without overt inflammation

HIGHLIGHTS

• who: Ananya Ananya from the The effects of combinations of polymeric nanoparticles, micelles, and various small molecule agonists on the activation of bone marrow derived DCs (BMDCs) harvested from the femur and tibia of aged (u2265, months) C BL/, male mice (n=6) were studiedBone marrow cells were cultured as outlined in Lutz et_al [101]. Briefly, cells were cultured in , mL RPMI , medium supplemented with , U/mL penicillin, mg/mL streptomycin, mM glutamine, % FBS, and , ng/ mL granulocyte-macrophage colony-stimulating factor (GM-CSF, Cat. #FB Z, Peprotech, Rocky Hill, NJ) at approximately , u00d7 u00ad, cells per . . .
  

   

  

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