HIGHLIGHTS
- who: Rongde Qiu and collaborators from the of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences, FEdward Hu00e9bert School of Medicine, Bethesda, MA, USA. have published the article: Kinesin-1 autoinhibition facilitates the initiation of dynein cargo transport, in the Journal: (JOURNAL) of 23/01/2023
- what: The kinAE178K-GFP mutant the authors intended to make contained an extra mutation (P426L), and the kinAE178K,E186K-GFP mutant also contained an extra mutation (K735R).
- how: For constructing the kinA-GFP fusion the authors used the following six oligos to amplify . . .
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