Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly

HIGHLIGHTS

• who: Jurica Matkoviu0107 from the Sample preparation, siRNA, plasmids, and dyes At, % confluence, DMEM medium was removed from the flask and cells were washed with , ml of phosphate buffered saline (PBS)Then, ml, % trypsin/ethylenediaminetetraacetic acid (EDTA, Biochrom AG, Berlin, Germany) was added to the flask and cells were incubated at , u00b0C and, % , in a humidified incubator for , min. After the incubation, trypsin was blocked by adding , ml of DMEM medium. For RNAi experiments, the cells were seeded to reach, % confluence the next day and cultured on , mm uncoated dishes withmm (#1., coverglass) glass thickness (MatTek Corporation . . .
  

   

  

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