L-selectin-mediated lymphocyte-cancer cell interactions under low fluid shear conditions*

HIGHLIGHTS

• who: Resto Vicente A. from the sas, VA)Cells were maintained in RPMI , medium containing glutamine supplemented with, % fetal bovine serum (FBS) under standard cell culture conditions. Human lymphocytes were purified from blood by isolation of the mononuclear cell fraction from peripheral blood (PBMCs). PBMCs were prepared by density gradient separation (Histopaque, Sigma) of peripheral blood obtained from healthy donors with full consent as per an Institutional Human Subjects Internal Review Board-approved protocol. Isolated PBMC were ⬎90% T cells (CD3⫹ cells) and expressed, selectin as determined by immunofluorescent staining and flow cytometry using a Beckman-Coulter . . .
  

   

  

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