Lack of nck1 protein and nck-cd3 interaction caused the increment of lipid content in jurkat t cells

HIGHLIGHTS

• who: Aussanee Nuiyen from the Nck, knocked-out Jurkat cells (N KO) were transfected with pEBB plasmid containing human Flag-tagged wildtype Nck-1, which was kindly provided by ProfBruce J. Mayer (Connecticut University Health Center, Farmington, CT, USA), as previously described [13]. After transfection, cells were cultured in , mL complete medium (RPMI , +, % FBS +, % Penicillin streptomycin +, % GlutaMAX) and incubated at , °C, % C ­ , for , h. Cell lysates were used to analyse Nck, expression level by western blotting using antibodies against Nck1, and GAPDH was used as the loading control. The PVDF membrane was carefully cut according to the . . .
  

   

  

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