Large-scale engineering of hipsc-derived nephron sheets and cryopreservation of their progenitors

HIGHLIGHTS

• who: Loes E. Wiersma from the All animal experimental protocols were approved by the animal welfare committee of the Leiden University Medical Center and the Dutch Animal Experiments CommitteeTo allow for transplantation of nephron sheets, a hollow punch (Renssteig) was used to create a , mm circular biopsy. These biopsies were transplanted at day , + , under the renal capsule of both kidneys in , seven-week-old recipient mice (non-obese diabetic/ severe combined immunodeficiency (NOD/SCID), Charles River Laboratories). Before sacrifice two mice were anesthetized with isoflurane and injected with low molecular mass , kDa dextran labelled with Tetramethylrhodamine (TRITC . . .
  

   

  

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