Leukemia inhibitory factor drives glucose metabolic reprogramming to promote breast tumorigenesis

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  • who: Xuetian Yue from the Glucose uptake assays Glucose uptake in cells was measured as previously described [21]Briefly, cells cultured in, well plates were pre-incubated in glucose-free medium for , min and then cultured for , min in medium containing , DG (, u00b5Ci/well). Cells were lysed in, % SDS. The radioactivity of cell lysates was measured by a liquid scintillation counter, and normalized to the protein concentrations of cell lysates. Assays for glucose uptake in xenograft tumors were performed as previously described [21]. Briefly, mice bearing xenograft tumors were injected (i.p.) with , DG (, u00b5Ci/g body . . .

     

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