Leukemia inhibitory factor (lif)-dependent, pluripotent stem cells established from inner cell mass of porcine embryos*

HIGHLIGHTS

• who: Bhanu Prakash V.L. Telugu from the tionsThey were differentiated into an endodermal lineage following , days of culture in the presence of a small molecule , (Stemgent) on a Matrigel substratum (31) and into neuronal lineage in the presence of , ng/ml FGF2, ng/ml FGF8, and , ng/ml Sonic hedgehog (RandD Systems) (32) on a laminin substratum. Teratoma Formation-pESOK II cells (, ⫻, ) were injected in, .25-ml volume with, % Matrigel (BD Biosciences) solution subcutaneously into two, month-old , nude mice (CD Foxn1nu, Charles River Laboratories). The derived tumors were dissected out and fixed in, % (v/v . . .
  

   

  

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