Lipid biosynthesis enzyme agpat5 in agrp-neurons is required for insulin-induced hypoglycemia sensing and glucagon secretion

HIGHLIGHTS

• who: Anastasiya Strembitska from the Mice Experiments were performed with, to, weeks old male and female miceAgpat5flox/flox mice were generated by genOway (genOway, Lyon, France) by homologous recombination in C BL6/N embryonic stem cells. A conditional knockout was created by flanking exon , of Agpat, with loxP sites, allowing for Cre-dependent recombination. Exon , contains a catalytic motif I sequence of Agpat, protein (HXXXXD) and its deletion results in out of frame splice and introduction of premature STOP codon in exon, . The resulting truncated mRNA product is unstable and no functional Agpat, protein is produced. Chimeric . . .
  

   

  

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