Longitudinal single-cell analysis of a patient receiving adoptive cell therapy reveals potential mechanisms of treatment failure

HIGHLIGHTS

• who: Rihao Qu from the With approval from the Yale University Institutional Review Board, using methods previously described, [2] single-cell RNA-sequencing (scRNA-seq) and TCRsequencing (TCR-seq) were conducted on TILs from the tumor harvested for ACT (baseline), circulating peripheral blood mononuclear cells (PBMCs) obtained the same day, the infused ACT product, TILs from resection of the progressing tumor removed in January, and PBMCs obtained concurrently (Fig A and supplemental materials). Clonotypes were identified by TCR-seq (supplemental materials), and transcriptomic analyses were conducted (supplemental materials.) Results, discussion and conclusions As shown in Fig. B . . .
  

   

  

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