Loss of the mitochondrial kinase pink1 does not alter platelet function

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  • who: from the Thrombin, adenosine diphosphate (ADP), prostaglandin e, (PGe1) and apyrase grade VII were from Sigma-Aldrich (Poole, UK)Cross-linked collagen related peptide (CRP) was purchased from Professor Richard Farndale (University of Cambridge, UK). Carbonyl cyanide, chlorophenylhydrazone (CCCP), Thromboxane mimetic, and the calcium ionophore, were from Tocris Bioscience (Bristol, UK). The , mimetic compound, was from Santa Cruz (Insight Biotechnology, Middlesex, UK). For western blotting, the antibodies were as follows: , (#BC100-494, :, dilution, NOVUS Biologicals, Abingdon, U.K.), (#sc-33796, :, dilution, Santa Cruz), (#S C and #S D, both, :, dilution, Dundee MRC PPU), (#6946, :, dilution), voltage . . .

     

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