Low throughput screening in neuroscience: using light to study central synapses one at a time

SUMMARY

    Other cellular neuroscientists, those with perhaps a more abstract mind, will rather intuitively converge to the iconic voltage traces of the action potential from Hodgkin and Huxley as the expression of the elemental essence of the brain in action. By allowing the activation of individual brain synapses with exquisite spatial and temporal control, two-photon (2P) uncaging of caged molecules provides a compelling illustration of substantial progress in the understanding of neural function afforded by the still expanding field of neurophotonics. Indeed, it is fairly straightforward to synthesize neurotransmitters and several methods have been developed over the . . .

     

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