Lssmscarlet2 and lssmscarlet3, chemically stable genetically encoded red fluorescent proteins with a large stokes’ shift

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SUMMARY

MCRISPRed is less stable at alkaline pH than at lower pH values since its fluorescence decreases at pH values higher than 7 with a pKa of 9.8. In this study, using the previously reported LSSmScarlet LSSRFP as a template, the authors developed pH-, photo-, and chemically stable LSSRFPs, named LSSmScarlet2 and LSSmScarlet3. LSSmScarlet2 and LSSmScarlet3 sustain their fluorescence in the range of pH from 2.5 to 10, although the molecular brightness of LSSmScarlet2 and LSSmScarlet3 is 1.3- to 1.5-fold lower than that of the parental LSSmScarlet. To investigate the . . .

 



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