Lung progenitors from lambs can differentiate into specialized alveolar or bronchiolar epithelial cells

HIGHLIGHTS

• who: Lung and colleagues from the Aliquots of , dissociated cells were labeled using an indirect , labeling technique with magnetic beads attached to a secondary antibodyBriefly, according to the manufacturer's instructions (Miltenyi Biotech), the cells were incubated with a, :, dilution of mouse anti-sheep , antibodies (clone Eq D , kindly provided by Pr CD Porada, University of Nevada) for , min on ice, washed in a buffer containing X PBS, mM EDTA and, .5% bovine serum albumin. The mixture was then incubated for , min on ice with goat anti-mouse IgG microbeads (Miltenyi France) at a, :, dilution in X . . .
  

   

  

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