Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models

HIGHLIGHTS

• who: Ying Xue Xie from the Generation of Tg-HALAMP Myc mice The transgenic mice were derived from a lentiviral vectorThe lentiviral vector included truncated human synapsin, promoter followed by ORF including , signal sequence-2xHA epitope tag-rat LAMP1- His-TEV cleavage site-2xMyc epitope tag. This lentivirus vector was digested at , °C with PacI and SphI to create akb linear fragment for pronuclear microinjection. The cleavage product included truncated, ′ LTR, the synapsin promoter and HALAMP Myc ORF (as described above), WPRE, and part of the bGH poly(A) signal. The enzymes were heat inactivated at , °C . . .
  

   

  

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