Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models

HIGHLIGHTS

• who: Ying Xue Xie from the Generation of Tg-HALAMP Myc mice The transgenic mice were derived from a lentiviral vectorThe lentiviral vector included truncated human synapsin, promoter followed by ORF including , signal sequence-2xHA epitope tag-rat LAMP1- His-TEV cleavage site-2xMyc epitope tag. This lentivirus vector was digested at , u00b0C with PacI and SphI to create akb linear fragment for pronuclear microinjection. The cleavage product included truncated, u2032 LTR, the synapsin promoter and HALAMP Myc ORF (as described above), WPRE, and part of the bGH poly(A) signal. The enzymes were heat inactivated at . . .
  

   

  

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