HIGHLIGHTS
- who: Akira Abe from the dishes followed by incubation at , °C in a humidified atmosphere of, % , in airAfter , h, non-adherent cells were removed by washing with phosphate-buffered saline. Ninety-five percent of alveolar lavaged cells and, % of peritoneal lavaged cells in the resultant adherent cell population were macrophages as confirmed by Wright-Giemsa staining (8). Greater than, % of the adherent peripheral blood mononuclear cells were monocytes (9). Isolation of Mouse Cells and Tissues-Wild-type C BL/, mice were obtained from Jackson Laboratory (Bar Harbor, ME). GM-CSF(⫺/⫺) was generated by Dranoff et_al (10). Bi . . .
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