Macromolecular crowding and decellularization method increase the growth factor binding potential of cell-secreted extracellular

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    Influence on the ECM for applications in tendon and tissue equivalents (Cigognini et_al, 2016; Tsiapalis et_al, 2020; Marinkovic et_al, 2021). Protein was separated using a 10% Bis-Tris Novex minigel (Invitrogen, Waltham, MA) and Coomassie blue stain and analyzed by nano LC/MS/MS with a Waters M-Class HPLC system interfaced to a ThermoFisher Fusion Lumos (MSBioworks, Ann Arbor, MI) (Mitra et_al, 2017; Harvestine et_al, 2018). Reconstituted ECM samples were prepared for scanning electron microscopy (SEM) following a standard hexamethyldisilazane dehydration process (Mitra et_al, 2017; Ramos-Rodriguez et_al, 2021). There is a critical . . .

     

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