HIGHLIGHTS
SUMMARY
Since the Mafa protein is not detectable by immunostainings in wild_type animals, but only in 4A homozygous mutants where the protein is stabilized, the authors compared (n=10 sections, from 2 animals/genotype) the number of Mafa+ neurons (immunostained for Mafa) in Mafa4A/4A to that of MafaLacZ/+, MafaLacZ/4A and MafaLacZ/LacZ (immunostained for βgal). Genomic DNA from G418 selected ES cells clones were screened by Southern Blot: DNA was digested by ScaI then, a 1.8 kb 3` external probe hybridized with either the recombinant Mafa flox4A (8.8 kb), the recombinant . . .
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