Manipulation of sterol homeostasis for the production of 24-epi-ergosterol in industrial yeast

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  • who: Yiqi Jiang from the Strains and media Yeast strains used in this study are listed in Supplementary Data Yeast strain , (CICC1746) is an industrial strain for ergosterol production and was obtained from the China Center of Industrial Culture Collection. , is kindly provided by Prof. Zhinan Xu (Zhejiang University, China). , genomic DNA was used for the amplification of ARE1, ARE2, YEH1, YEH2, and TGL1. Escherichia coli Trans-, (TransGen Biotech, China) was used as the host to construct, maintain, and amplify plasmids. E. coli strains were cultured in LB medium with have published the research: Manipulation of sterol . . .

     

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