Mapping the binding site of the p2x receptor antagonist ppads reveals the importance of orthosteric site charge and the cysteine-rich head region

HIGHLIGHTS

• who: Hong Huo from the Molecular biology The hP , receptor cDNA was originally cloned from the bladder (23) and the rP , receptor DNA was a gift of DrFrancois Rassendren (CNRS, Montpellier, France). A mutation (Y A) was introduced to the rP , receptor template to give more stable and reproducible currents (24). Point mutations were made using the QuikChange mutagenesis kit (Stratagene). Mutations were verified by DNA sequencing by the Protein and Nucleic Acid Chemistry Laboratory services, University of Leicester. Expression in Xenopus laevis oocytes cRNA was synthesized using the , mMessage mMachine kit (Ambion) and injected into stage . . .
  

   

  

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