Marek’s disease vaccines-induced differential expression of known and novel micrornas in primary lymphoid organ bursae of white leghorn

HIGHLIGHTS

• who: Lei Zhang from the Total RNA samples were quantitatively and qualitatively checked with a NanoDrop , Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and an Agilent , Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), respectivelyGood quality RNA samples were chosen to construct standard cDNA libraries using Illumina TruSeq Small RNA Library Preparation kits following the manufacturer's recommendations. Completed libraries were subjected to routine quality control (QC) checks and quantified using a combination of Qubit dsDNA HS and Agilent Bioanalyzer High Sensitivity DNA assays. The libraries were sequenced on an Illumina HiSeq , sequencer using SBS (Sequencing by Synthesis . . .
  

   

  

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