Marker-free carotenoid-enriched rice generated through targeted gene insertion using crispr-cas9

HIGHLIGHTS

  • who: Oliver Xiaoou Dong from the University of have published the article: Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS
  • what: The authors demonstrate the targeted insertion of a 5.2 kb marker-free DNA fragment at two GSHs in rice using CRISPR-Cas, and obtain homozygous carotenoidenriched rice.
  • how: To identify GSHs in rice the authors conducted a mutant screen by analyzing the morphological records and the whole-genome sequencing data of a fast-neutron rice mutant collection in a . . .

     

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