Marker-free co-selection for successive rounds of prime editing in human cells

HIGHLIGHTS

• who: Su00e9bastien Levesque from the (UNIVERSITY) have published the paper: Marker-free co-selection for successive rounds of prime editing in human cells, in the Journal: (JOURNAL) of 27/09/2022
• what: The authors design a robust co-selection strategy through coediting of the ubiquitous and essential sodium/potassium pump (Na+/K+ ATPase). The authors propose an easy-to-implement toolkit to select cells engineered via PE without the use of exogenous selection markers that largely bypasses the need for extensive pegRNA optimization. Primers used in this study and the PCR product sizes are provided . . .
  

   

  

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