HIGHLIGHTS
- who: Jingqi Liu and colleagues from the University of Bristol, United Kingdom have published the Article: Mechanism of leptin-NPY on the onset of puberty in male offspring rats after androgen intervention during pregnancy, in the Journal: (JOURNAL)
- how: For the detection of RNA concentration the authors used 1 mg total RNA reverse transcription cDNA according to the instructions of the kit manufacturer (Thermo Scientific) and the mRNA levels of AR ERa NPY leptinR and NPY2R in the above tissues were detected by quantitative real-time PCR. The results showed that the protein expression levels . . .
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