Mechanistic basis for catalytic activation of mitogen-activated protein kinase phosphatase 3 by extracellular signal-regulated kinase*

HIGHLIGHTS

• who: Clark C. Fjeld from the (UNIVERSITY) have published the paper: Mechanistic Basis for Catalytic Activation of Mitogen-activated Protein Kinase Phosphatase 3 by Extracellular Signal-regulated Kinase*, in the Journal: (JOURNAL)
• what: The authors provide detailed biochemical evidence that ERK activates MKP3 through the stabilization of the active phosphatase conformation, inducing closure of the catalytic "general acid" loop. Using steady-state and rapidreaction kinetics, pH studies, and Brönsted analysis, the authors demonstrate that ERK-activated MKP3 utilizes general acid catalysis, enhancing the rate of P-O bond cleavage by 100-fold.

 

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