Mechanistic insights of abc importer hutcd involved in heme internalization by vibrio cholerae

HIGHLIGHTS

• who: Indrila Saha from the Department have published the paper: Mechanistic insights of ABC importer HutCD involved in heme internalization by Vibrio cholerae, in the Journal: Scientific Reports Scientific Reports
• how: The authors therefore decided to investigate the interactions of AMP.PNP the non-hydrolysable ATP analog with HutD by monitoring the intrinsic fluorescence quenching at an excitation wavelength of 280 nm (u03bbexc=280 nm u03bbem=295-400 nm) accounting the contributions of tryptophans and tyrosines present at the ligand binding_site.

SUMMARY

To identify the oligomeric state of HutD, crosslinking experiments . . .

 



If you want to have access to all the content you need to log in!

Thanks :)

Username or Email

Password

Remember me

Lost your password?

If you don't have an account, you can create one here.