Mechanistic studies in drosophila and chicken give new insights into functions of dvl1 in dominant robinow syndrome

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• who: JNK) Wnt signaling. and colleagues from the The open reading frame encoding human , (Origene RC217691) was originally in the p, vector (Origene-provided clone in this vector) with the full coding sequence and two tags, myc and FlagWe moved , into p, (Invitrogen) using Gateway cloning. All site-directed mutagenesis was performed in those clones and then recombined into the pENTRY destination vector (Invitrogen) with LRclonase, (Life Technologies). Three mutations that cause autosomal dominant RS type II were generated (OMIM:, ) using site-directed mutagenesis with restriction-free cloning (Bond and Naus, ): the, u0394T, u0394G and, u0394A mutations . . .
  

   

  

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