Mechanosensitive piezo1 calcium channel activates connexin 43 hemichannels through pi3k signaling pathway in bone

HIGHLIGHTS

• who: Yan Zeng from the University School of MedicineLentiviral-mediated CRISPR/Cas, genome editing technology using lentiCRISPRv2:Cx43-sgRNA or ROSA26-sgRNA was applied to generate Cx, KD and Rosa, control , cells as previously described [26]. These cells were cultured on collagen-coated (rat tail collagen type I,mg/ml) surfaces and were grown in medium (u03b1-MEM, u00adHCO3-) supplemented with, .5% fetal bovine serum and, .5% bovine calf serum and incubated in a, % u00ad, incubator at , u00b0C as described previously [27]. Fluid flow shear stress and in vitro dye uptake assay have published the Article: Mechanosensitive . . .
  

   

  

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